By Sunil Bhardwaj


Paper Chromatography was first employed by scientist Mikhail Tsvet in 1900. In 1952 A. Martin and R. Synge won the Noble prize in chemistry for the development of other Chromatographic techniques.

In paper chromatography the paper is important component. Several types of papers of different size, shapes, porosity and chemically treated are available in the market. Paper is randomly oriented cellulose fiber, cellulose is hydrophilic means solvent loving. Water or other polar solvents are tightly held by cellulose.

The following are the important applications of chromatograph;

1) Paper Chromatography is widely used in the analysis of organic, inorganic and Biochemical compounds.

2) The detection of Al and Cr in the metal ore can be done by this phenomenon.

3) Organic spotting can be done by paper chromatography.

4) In semi-micro qualitative analysis, the metal ions present in the same group can be done simultaneously.

5) With the help of this chromatography, we can study ripening and Fermentation phenomenon.

6) We can test purity of sample. Pure compound give one spot.

7) We can detect the drug and dopes present in the animal and human body.

8) Detection and estimation of adulates, and contaminants in the food material and in the drinks can be done.

9) The phenomenon is used in pathological laboratory for examination of amino acids and sugar present in urine and in other fluids of the body.

10) The phenomenon can be used for the analysis of corrosive reagents.

11) Phenomenon is used in the analysis of cosmetics.


1) The development of chromatogram requires long time about 24 hours. So it is time consuming. So the process is slow.

2) It does not give accurate results for larger concentrations.

3) Sensitivity of the method is low.

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