When monochromatic light is incident on molecule, molecule absorb the radiations transition of the molecules take place and those molecules come back instantaneously in original state with the Emission of the radiations which is in the form of scattered light, that scattered light give the spectrum. In that spectrum some lines are those which have frequency less than incident and have frequency more than incident. While some are having same frequency as that of incident. One which has less are called stoke lines, which are having more are called Antistoke lines, and that which has same are Rayleigh. The formation of stokes and antistokes lines are known as Raman spectra.
1) With the help of Raman spectra we can analyze homo nuclear molecules for e.g. H2, N2, and O2 etc very accurately.
2) Tri atomic molecules for e.g. H2O, SO2, NO2, CO2, CS2 etc can be studied by Raman spectra and structural nature for e g linear, angular, symmetric, asymmetric etc.
3) With the help of Raman Spectra we can get the idea about the binding forces of atoms in a lattice and the strength and the nature of the forces which hold the crystal together.
4) It helps to study the phenomenon of electrolytic dissociation and degree of hydrolysis acids, bases and salts.
5) With the help of Raman spectra following groups can be identified in molecules OH-, NO3-, SO4-2, PO4-3, ClO4-, etc.
6) Open chain compounds can be classified separately from closed Chain compounds with the help of Raman spectra.
7) The number of molecule of water of crystallization present in the compound can be determined with the help Raman spectra.
8) With the help of Raman spectra isomers can be identified
9) With the help of Raman spectra we can find out the presence of specific linkage in a molecule.
10) Transition from crystalline to amorphous state can be studied with the help of Raman spectra.
11) Complex compounds can be studied with the help of Raman spectroscopy.
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